# Rice Half-MS medium | Chemicals | Stock
Conc. | Addition | Final
Conc. | Location | | :-------- | :---: | :---: | :---: | :---: | | MS basal salt ( M407 ) | — | 1.22 g | 0.5X | 4°C ( c ) | | Ammonium nitrate ( NH₄NO₃ ) | — | 3.30 g | 10.31 mM | Ⅷ | | Potassium nitrate ( KNO₃ ) | — | 3.80 g | 9.40 mM | toxic (D) | | Potassium phosphate monobasic ( KH₂PO₄ ) | 170 g / L | 2 mL | 0.63 mM | bench | | MES hydrate | — | 2 g | 0.05% (w/v) | Ⅲ | | PPM™ | 100% | 2 mL | 0.05% (v/v) | 4°C ( a ) | | KOH ^※ | 5 M | ~ 730 µL | — | Ⅶ | | ddH₂O | — | ~ 4 L | — | — | | **Total volume** | | **4 L** | | | Gelling agent ⟶ Gelzan™ ( G3251, CAS no.: 71010-52-1 ) : 1.5 g / L ( 0.15% ). Located between Ⅰ and Ⅱ. Generally, we make 400 mL per bottle, so weigh ( 600 ± 2 ) mg / 400 mL for each bottle. The final pH should be around 5.7 ~ 5.8. Autoclave under 121°C for 20 mins. ^※ KOH is used for pH adjustment. To prepare 5 M KOH solution, dissolve 330 g of 85% KOH in 900 mL ddH₂O, and then bring to 1 L. Since KOH releases heat during dissolution, gradually and slowly add the KOH granules into the water.