| Chemicals | Stock Conc. |
Addition | Final Conc. |
Location |
|---|---|---|---|---|
| MS basal salt ( M407 ) | — | 1.22 g | 0.5X | 4°C ( c ) |
| Ammonium nitrate ( NH4NO3 ) | — | 3.30 g | 10.31 mM | Ⅷ |
| Potassium nitrate ( KNO3 ) | — | 3.80 g | 9.40 mM | toxic (D) |
| Potassium phosphate monobasic ( KH2PO4 ) | 170 g / L | 2 mL | 0.63 mM | bench |
| MES hydrate | — | 2 g | 0.05% (w/v) | Ⅲ |
| PPM™ | 100% | 2 mL | 0.05% (v/v) | 4°C ( a ) |
| KOH ※ | 5 M | ~ 730 µL | — | Ⅶ |
| ddH2O | — | ~ 4 L | — | — |
| Total volume | 4 L |
Gelling agent ⟶ Gelzan™ ( G3251, CAS no.: 71010-52-1 ) : 1.5 g / L ( 0.15% ). Located between Ⅰ and Ⅱ.
Generally, we make 400 mL per bottle, so weigh ( 600 ± 2 ) mg / 400 mL for each bottle.
The final pH should be around 5.7 ~ 5.8. Autoclave under 121°C for 20 mins.
※ KOH is used for pH adjustment. To prepare 5 M KOH solution, dissolve 330 g of 85% KOH in 900 mL ddH2O, and then bring to 1 L.
Since KOH releases heat during dissolution, gradually and slowly add the KOH granules into the water.