Rice seed germination

Step 1: Homogenization

1. Dehusked the seeds. Select only seeds that are well-developed, fully-filled, clean, and transparent.

2. Dry-incubate the seeds in dark under 45°C for 24 hours.

Step 2: Sterilization

1. Soak the seeds in 3% bleach solution ( add one drop of detergent ) and gently inverting / shaking for 20 mins.

2. Wash at least 6 times with ddH₂O, until the bubble has gone and water is clear.

Step 3: Priming and imbibition

1. Remove the water thoroughly with pipette.

2. (optional) Seed priming. Soak the seeds in 3% KNO₃ solution under room temperature for 24 hours;

• The KNO₃ priming method is not effective in our case

3. Soak the seeds in 1 ~ 2% Plant Preservative Mixture™ ( PPM™ ) solution and incubate at 30°C in dark for 16 ~ 24 hours.

• For example, add 300 µL PPM into 30 mL sterilized ddH₂O.

Step 4: Sowing

1. Remove the PPM solution.

2. Sow the seeds on suitable medium.

• The seed must be fully embedded into the medium, otherwise, the seedling will push itself up during root penetrating into the gel.
• Optional: Add 0.05 ~ 0.2% ( v / v ) PPM™ to the medium, if it is difficult to control contamination. The PPM™ can be autoclaved together with the media.

Done !!!